AlphaScreen SureFire™ ERK Assay Kit

An alternative to calcium or cAMP measurement for cellular assay of GPCR activation utilizing PerkinElmer’s AlphaScreen technology and TGR BioScience’s SureFire™ cellular ERK assay

Key Features & Benefits

  • Homogeneous cellular assay for ERK 1/2:
    No-wash, non-isotopic, miniaturizable cel-based functional HTS assay for almost any GPCR including Gaq-, many Gai/o- and some Gas-coupled receptors.

  • Ideally suited for Gaq- & Gai-coupled receptors:
    Permits robust screening of high interest but traditionally challenging Gaq- & Gai-coupled GPCR targets.

  • Excellent for “tough-to-screen” GPCR targets:
    Well suited for ‘tough’ targets not optimally coupled through the calcium or cAMP pathways.

  • Suitable for over-expressed and endogenous receptors:
    Extremely sensitive assay which can be used with cells over-expressing cloned targets or with cells expressing physiologically relevant endogenous levels of receptor target.

  • Use in any cell line, including primary cells:
    Flexible assay format and application use.

  • Easy to scale up and automate with excellent Z’ values:
    Utilizes HTS-proven AlphaScreen technology, which is easily scalable to 384- and 1536-well microplate formats, while yielding outstanding S/Bs and Z’ values.

For more information go to the microsite product pages, or you can download important product collateral materials from the links below:



Visit TGR BioSciences website

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Analysis of Gαq- Coupled GPCRs

Analysis of Gq Coupled GPCRs

Gαq-coupled human Bradykinin R2 receptor was overexpressed in HEK 293 cells and stimulated with various concentrations of Bradykinin ligand. Phospho-ERK was measured in whole cell lysates using the AlphaScreen SureFire™ ERK assay.

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Analysis of Gαi-Coupled GPCRs

Analysis of Gi Coupled GPCRs

Gαi-coupled human Muscarinic M4 receptor was overexpressed in CHO cells and stimulated with various concentrations of Carbachol ligand. Phospho-ERK was measured in whole cell lysates using the AlphaScreen SureFire™ ERK assay.

 

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Analysis of Endogenously Expressed GPCRs

Analysis of Endogenously Expressed GPCRs

Gαq-coupled mouse Thrombin receptor, expressed at endogenous levels in Swiss 3T3 embryonic fibroblast cells, was stimulated with various concentrations of Thrombin ligand. Phospho-ERK was measured in whole cell lysates using the AlphaScreen SureFire™ ERK assay.

 

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Upcoming Trade Shows Featuring AlphaScreen SureFire™ ERK Assay

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©2006 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. AlphaScreen is a trademark of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. SureFire™ is a registered trademark of TGR BioSciences. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.